Procedures for Control Testing
1. Take out appropriately colored glow stick and break it to mix the chemicals.
2. Tie the glow stick to the bar over the testing tank and add weights to make it sink to the bottom.
3. Collect five shrimp from the control tank and place them in a bucket with water from the testing tank for fifteen minutes to allow them to adjust.
4. Prepare the stopwatch and place them in the testing tank.
5. Record the data.
6. Collect the shrimp after testing is complete and place them back into the control tank.
7. Let them readjust to the tank and repeat steps 1-6 until finished with testing.
Procedures for Testing Low Phosphorus
1. Take out appropriately colored glow stick and break it to mix the chemicals.
2. Tie the glow stick to the bar over the testing tank and add weights to make it sink to the bottom.
3. Collect five shrimp from the tank with low amounts of phosphorus and place them in a bucket with water from the testing tank and water from the tank with low phosphorus for fifteen minutes to allow them to adjust.
4. Prepare the stopwatch and place them in the testing tank.
5. Record the data.
6. Collect the shrimp after testing is complete and place them back into the appropriate tank.
7. Let them readjust to the tank and repeat steps 1-6 until finished with testing.
Procedures for Testing Medium Phosphorus
1. Take out appropriately colored glow stick and break it to mix the chemicals.
2. Tie the glow stick to the bar over the testing tank and add weights to make it sink to the bottom.
3. Collect five shrimp from the tank with medium amounts of phosphorus and place them in a bucket with water from the testing tank and water from the tank with medium phosphorus for fifteen minutes to allow them to adjust.
4. Prepare the stopwatch and place them in the testing tank.
5. Record the data.
6. Collect the shrimp after testing is complete and place them back into the appropriate tank.
7. Let them readjust to the tank and repeat steps 1-6 until finished with testing.
Procedures for Testing High Phosphorus
1. Take out appropriately colored glow stick and break it to mix the chemicals.
2. Tie the glow stick to the bar over the testing tank and add weights to make it sink to the bottom.
3. Collect five shrimp from the tank with high amounts of phosphorus and place them in a bucket with water from the testing tank and water from the tank with high phosphorus for fifteen minutes to allow them to adjust.
4. Prepare the stopwatch and place them in the testing tank.
5. Record the data.
6. Collect the shrimp after testing is complete and place them back into the appropriate tank.
7. Let them readjust to the tank and repeat steps 1-6 until finished with testing.
Safety Procedures for Sea Salt
1. The use of sea salt will used in accordance with the MSDS
2. Using sea salt will be done with gloves, aprons, and goggles.
3. A fire blanket, fume hood fire extinguisher, shower and eyewash station are available for use.
4. According to the MSDS excess salt will rinsed down the sink with 10 fold excess water.
5. After using sea salt, hands will be washed with soap and water.
Safety Protocol for pH Buffer Calcium Carbonate
1. The use of Calcium carbonate will used in accordance with the MSDS
2.Using calcium carbonate will be done with gloves, aprons, and goggles.
3. A fire blanket, fume hood fire extinguisher, shower and eyewash station are available for use.
4. According to the Flinn catelogue, excess calcium carbonate will wrapped in newspaper, placed in a cardboard box, and disposed of in a landfill.
5. After using calcium carbonate, hands will be washed with soap and water.
Tank Set Up
1. Obtain 4 ten-gallon tanks and one bigger tank.
2. Fill each tank with tap water and let sit for one week to allow the chlorine to evaporate.
3. Add 1 inch of gravel to the bottom of each tank.
4. Place a water filter on each tank and check that the spinner does not clog or stop on a daily basis.
5. Add two shrimp to the tank to help the nitrogen cycle.
6. Check the pre-filter and clean it once a week.
7. Change and remove the carbon filter every month.
8. Every month change 10% of the water with clean fresh DO water.
9. Check the pH, salt level, and temperature every week with the Hanna pH EC/TDS HI98130 probe.
* a. Press and hold the mode button to turn on.
* b. Select the item to be tested with the set/hold button.
* c. Submerge the probe into the tank to be tested.
* d. When the stability clock disappears the measurement can be taken.
* e. Be sure to calibrate the probe before each test.
* f. Turn off the probe when done testing
Adjusting the Tanks
1. Identify the water level to be about 2-3 cm below the black plastic lip of the tank.
2. Add reversed Osmosis water to replace evaporated water.
3. To adjust the pH small amounts of vinegar will be used to adjust for acidic environment and small amounts of baking soda (sodium bicarbonate) will be used to create a basic environment. (pH-up and pH down can also be used.)
4. In the event that salinity levels change more water will be used to dilute the levels and more marine salt will be added to increase salinity.
Inserting Living Organisms
1. Place the container or bag in which the Pink Atlantic shrimp came in into the tank for 15 to 30 minutes. During this time they can be acclimated to the water temperature.
2. Add half of the tank water to the bag and reinsert the bag into the tank for another 15 to 30 minutes.
3. Release the Pink Atlantic shrimp and water into the tank.
4. Do not feed the Pink Atlantic Shrimp for a day allowing it to get used to the environment.
1. The use of sea salt will used in accordance with the MSDS
2. Using sea salt will be done with gloves, aprons, and goggles.
3. A fire blanket, fume hood fire extinguisher, shower and eyewash station are available for use.
4. According to the MSDS excess salt will rinsed down the sink with 10 fold excess water.
5. After using sea salt, hands will be washed with soap and water.
Safety Protocol for pH Buffer Calcium Carbonate
1. The use of Calcium carbonate will used in accordance with the MSDS
2.Using calcium carbonate will be done with gloves, aprons, and goggles.
3. A fire blanket, fume hood fire extinguisher, shower and eyewash station are available for use.
4. According to the Flinn catelogue, excess calcium carbonate will wrapped in newspaper, placed in a cardboard box, and disposed of in a landfill.
5. After using calcium carbonate, hands will be washed with soap and water.
Tank Set Up
1. Obtain 4 ten-gallon tanks and one bigger tank.
2. Fill each tank with tap water and let sit for one week to allow the chlorine to evaporate.
3. Add 1 inch of gravel to the bottom of each tank.
4. Place a water filter on each tank and check that the spinner does not clog or stop on a daily basis.
5. Add two shrimp to the tank to help the nitrogen cycle.
6. Check the pre-filter and clean it once a week.
7. Change and remove the carbon filter every month.
8. Every month change 10% of the water with clean fresh DO water.
9. Check the pH, salt level, and temperature every week with the Hanna pH EC/TDS HI98130 probe.
* a. Press and hold the mode button to turn on.
* b. Select the item to be tested with the set/hold button.
* c. Submerge the probe into the tank to be tested.
* d. When the stability clock disappears the measurement can be taken.
* e. Be sure to calibrate the probe before each test.
* f. Turn off the probe when done testing
Adjusting the Tanks
1. Identify the water level to be about 2-3 cm below the black plastic lip of the tank.
2. Add reversed Osmosis water to replace evaporated water.
3. To adjust the pH small amounts of vinegar will be used to adjust for acidic environment and small amounts of baking soda (sodium bicarbonate) will be used to create a basic environment. (pH-up and pH down can also be used.)
4. In the event that salinity levels change more water will be used to dilute the levels and more marine salt will be added to increase salinity.
Inserting Living Organisms
1. Place the container or bag in which the Pink Atlantic shrimp came in into the tank for 15 to 30 minutes. During this time they can be acclimated to the water temperature.
2. Add half of the tank water to the bag and reinsert the bag into the tank for another 15 to 30 minutes.
3. Release the Pink Atlantic shrimp and water into the tank.
4. Do not feed the Pink Atlantic Shrimp for a day allowing it to get used to the environment.
Chemical Safety:
1. During the part of the experiment that will be performed at the school, all chemicals will be used in accordance with the MSDS.
2. The use of all chemicals will be done while wearing gloves, aprons, and goggles.
3. All chemicals that release fumes will be placed under the fume hood to prevent the release of fumes into the classroom.
4. A fire blanket, fume-hood, fire extinguisher, eyewash station, and shower are available for use.
5. Upon leaving the lab, hands will be washed and all chemicals will be stored in a locked ventilated chemical storeroom.
1. During the part of the experiment that will be performed at the school, all chemicals will be used in accordance with the MSDS.
2. The use of all chemicals will be done while wearing gloves, aprons, and goggles.
3. All chemicals that release fumes will be placed under the fume hood to prevent the release of fumes into the classroom.
4. A fire blanket, fume-hood, fire extinguisher, eyewash station, and shower are available for use.
5. Upon leaving the lab, hands will be washed and all chemicals will be stored in a locked ventilated chemical storeroom.
Disposal
Waste disposal must be in accordance with appropriate Federal, State, and local regulations. This product, if unaltered by use, may be disposed of by treatment at a permitted facility or as advised by your local hazardous waste regulatory authority. Residue from fires extinguished with this material may be hazardous.
